Anti-alpha-Tubulin Purified

• Catalog Number: orb738510
• Category: Antibodies
• Description: Mouse monoclonal antibody to TUBA1
• Clonality: Monoclonal
• Clone Number: YOL1/34
• Tested applications: ELISA, FC, WB
• Reactivity: Aves, Human, Mammal, Mouse, Rat, Yeast
• Isotype: Rat IgG2a
• Immunogen: Yeast tubulin
• Concentration: 1 mg/ml
• Dilution range: ICC: 4-8 μg/ml, WB: 1 μg/ml
• Purity: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated
• Target: alpha-Tubulin
• Entrez: 7277
• UniProt ID: Q71U36
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TUBA antibody
• Note: For research use only
• Application notes: Immunocytochemistry: Recommended dilution 4-8 μg/ml.Western blotting: Recommended dilution 1-2 μg/ml; reducing conditions.Flow cytometry: Recommended dilution: 8-12 μg/ml; intracellular staining.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).

Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).