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Anti-alpha-Tubulin FITC
Catalog Number: orb44531
| Catalog Number | orb44531 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to alpha tubulin. |
| Clonality | Monoclonal |
| Clone Number | TU-01 |
| Tested applications | FC, ICC |
| Reactivity | Aves, Human, Invertebrate, Mouse, Paramecium, Plant, Porcine, Yeast |
| Isotype | Mouse IgG1 |
| Immunogen | Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. |
| Concentration | 1 mg/ml |
| Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | FITC |
| Target | alpha-Tubulin |
| Entrez | 7277 |
| UniProt ID | Q71U36 |
| RRID | AB_10996753 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-alpha-tubulin antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.
Separation of HeLa cells stained using anti-human alpha-tubulin (TU-01) FITC antibody (concentration in sample 5 μg/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 5 μg/ml, same as alpha-tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.
