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Anti-ALOX12 Antibody
Catalog Number: orb1819382
| Catalog Number | orb1819382 |
|---|---|
| Category | Antibodies |
| Description | Anti-ALOX12 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | E.coli-derived human ALOX12 recombinant protein (Position: A186-R628). Human ALOX12 shares 87.3% and 86% amino acid (aa) sequence identity with mouse and rat ALOX12, respectively. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 75 kDa |
| UniProt ID | P18054 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | 70 kDa ribosomal protein S6 kinase 1 antibody, KS6 Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of ALOX12 using anti-ALOX12 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX12 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALOX12 at approximately 75 kDa. The expected band size for ALOX12 is at 75, 80 kDa.
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