Anti-AKT1 Monoclonal Antibody

• Catalog Number: orb546987
• Category: Antibodies
• Description: Anti-AKT1 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: EBG-1
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
• Antibody Type: Primary Antibody
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• UniProt ID: P31749
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Signal transducer and activator of transcription 3
• Note: For research use only
• Application notes: WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Expiration Date: 12 months from date of receipt.

WB analysis of AKT1 expression in MCF-7 Cell.

WB analysis of NOTCH1 expression in (1) HepG2 Cell; (2) A549 Cell; (3) HeLa Cell; (4) Raji Cell; (5) MCF-7 Cell; (6) SiHa Cell; (7) 293T Cell.

WB analysis of NOTCH1 expression in (1) Rat liver Tissue; (2) Rat brain Tissue; (3) Rat C6 Cell; (4) Rat RH35 Cell; (5) Mouse liver Tissue; (6) Mouse brain Tissue; (7) Mouse Neuro-2a Cell; (8) Mouse HEPA1-6 Cell.

Immunofluorescent analysis of HeLa cells, using AKT1 Antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.