Anti-ADAMTS13 Antibody

• Catalog Number: orb381030
• Category: Antibodies
• Description: Anti-ADAMTS13 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ADAMTS13 recombinant protein (Position: A299-R488). Human ADAMTS13 shares 85.2% amino acid (aa) sequence identity with mouse ADAMTS13.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 154 kDa
• UniProt ID: Q76LX8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: A disintegrin and metalloproteinase with thrombosp
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat ELISA(Cap) , 0.1-0.5μg/ml, Human, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Sandwich ELISA - Recombinant human ADAMTS13 protein standard curve. Use in combination with reagents from Human ADAMTS13 ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: rat brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS13 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAMTS13 at approximately 154 KD. The expected band size for ADAMTS13 is at 154 KD.

Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Western blot analysis of ADAMTS13 expression in human HepG2 whole cell lysates 50 ug, ADAMTS13 at 154 KD under non-reducing conditions and 200 kD under reducing conditions were detected using rabbit anti-ADAMTS13 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.