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Anti-14-3-3 alpha + beta YWHAB Rabbit Monoclonal Antibody
Catalog Number: orb548041
| Catalog Number | orb548041 |
|---|---|
| Category | Antibodies |
| Description | Anti-14-3-3 alpha + beta YWHAB Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Monoclonal |
| Clone Number | HHA-25 |
| Tested applications | FC, ICC, IF, IHC, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human 14-3-3 alpha + beta |
| Antibody Type | Primary Antibody |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 80 kDa |
| UniProt ID | P31946 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | 14-3-3 protein beta/alpha; Protein 1054; Protein k Read more... |
| Note | For research use only |
| Application notes | WB 1:500-1:1000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:30FC 1:30. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of 14-3-3 alpha + beta expression in HeLa lysate (orb548041). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody (Catalog # orb548041) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for YWHAB.
