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Catalog Number | orb781176 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ghrelin(GHRL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ghrelin(GHRL). |
Tested applications | ELISA |
Reactivity | Bovine |
Range | 156.25-10000 pg/mL |
Concentration | 10000 pg/mL |
UniProt ID | Q9BDJ6 |
Sensitivity | 51.5 pg/mL |
Protein Length | 2h |
Alternative names | MTLRP ELISA Kit, Growth Hormone-Releasing Peptide Read more... |
Note | For research use only |
Application notes | standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GHRL in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |