You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
ANGPTL4 Antibody (Center)
Catalog Number: orb1928044
| Catalog Number | orb1928044 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB18264 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 45214 Da |
| Target | This ANGPTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the Central region of human ANGPTL4. |
| UniProt ID | Q9BY76 |
| NCBI | NP_001034756.1, NP_647475.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Angiopoietin-related protein 4, Angiopoietin-like Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ANGPTL4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-ANGPTL4 Antibody (Center) at 1:1000 dilution. Lane 1: human skeletal muscle lysate. Lane 2: human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A549 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
