Angiotensin II type 1A receptor Rabbit Polyclonal Antibody

• Catalog Number: orb13241
• Category: Antibodies
• Description: Angiotensin II type 1A receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Angiotensin II type 1A receptor (101-200/359aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: AGTR1
• UniProt ID: P30556
• RRID: AB_10752182
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AGTR1; Agtr1a; AT1; AT1A; AT1AR; Type 1 angiotensi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (orb13241) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (orb13241) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A549 (Human) Cell Lysate at 430 ug, Primary: Anti-Angiotensin II type 1A receptor (orb13241) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41/51 kD.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Placenta (Mouse) Lysate at 40 ug, Lane 4: Kidney (Mouse) Lysate at 40 ug, Lane 5: Embryo (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Mouse) Lysate at 40 ug, Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 ug, Lane 8: Liver (Rat) Lysate at 40 ug, Lane 9: Heart (Rat) Lysate at 40 ug, Lane 10: Kidney (Rat) Lysate at 40 ug, Lane 11: Cerebrum (Rat) Lysate at 40 ug, Lane 12: Embryo (Rat) Lysate at 40 ug, Primary: Anti-Angiotensin II type 1A receptor (orb13241) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 46 kD.

Sample: Liver (Mouse) Lysate at 40 ug, Lung (Mouse) Lysate at 40 ug, Adrenal gland (Rat) Lysate at 40 ug, Primary: Anti-Angiotensin II type 1A receptor (orb13241) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 56 kD.