AMPH Antibody

• Catalog Number: orb1248023
• Category: Antibodies
• Description: AMPH Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-KRKDESRISKAE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). Recommended concentration: 0.1-0.5ug/ml. A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: AMPH
• UniProt ID: P49418
• NCBI: NP_001626.1, NP_647477.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). Recommended concentration: 0.1-0.5ug/ml. A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248023 (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248023 (2.5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.