Aminoallyl-dUTP - Solid

SKU: orb533130

Description

Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerase in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• CAS Number: 116840-18-7
• MW: Theoretical MW: 523.22 g/mol (free acid); Detected MW: 523.02 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₁₂H₂₀N₃O₁₄P₃
• SMILES: NC/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only