AMBRA1 antibody

• Catalog Number: orb377918
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AMBRA1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human AMBRA1
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: AMBRA1
• Entrez: 228361, 55626
• UniProt ID: Q9C0C7, A2AH22
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: KIAA1736; Activating molecule in BECN1-regulated a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMBRA1 expression in MCF7 (A), A431 (B) whole cell lysates. (Predicted band size: 84; 132; 135; 139; 142 kD; Observed band size: 143 kD)

Immunohistochemical analysis of AMBRA1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.