AMACR / p504S Antibody (Prostate Cancer Marker)

• Catalog Number: orb639955
• Category: Antibodies
• Description: This antibody recognizes a protein of 42kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and b-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: AMACR/4572R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Full length protein was used as the immunogen for the recombinant AMACR antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant AMACR antibody is available for research use only.
• UniProt ID: Q9UHK6
• Storage: Store the recombinant AMACR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma with recombinant AMACR antibody. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.

IHC testing of FFPE human prostate carcinoma with recombinant AMACR antibody. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.

Western blot testing of human kidney lysate with recombinant AMACR antibody. Predicted molecular weight ~43 kDa.