AMACR / p504S Antibody (Prostate Cancer Marker)

• Catalog Number: orb534283
• Category: Antibodies
• Description: The AMACR gene encodes the racemase Alpha-methylacyl-CoA. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [RefSeq]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2MACR-1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 297-394 was used as the immunogen for the AMACR antibody.
• Dilution range: Western blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This AMACR antibody is available for research use only.
• UniProt ID: Q9UHK6
• Storage: Store the AMACR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the AMACR antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human kidney lysate with AMACR antibody (clone 2MACR-1). Predicted molecular weight ~43 kDa.

IHC testing of FFPE human prostate carcinoma with AMACR antibody (clone 2MACR-1). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.