Alpha Synuclein Antibody: Biotin

• Catalog Number: orb536155
• Category: Antibodies
• Description: Rabbit polyclonal antibody against alpha Synuclein conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant Human alpha Synuclein Fibril
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• MW: 14.46kDa
• Target: Alpha Synuclein
• Entrez: 6622
• UniProt ID: P37840
• NCBI: NP_000336.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Alpha Synuclein antibody, Non-A4 component of amyl
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-800 was sufficient for detection of Alpha Synuclein in 15 μg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Tissue: Cerebellum. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody.

Western blot analysis of Mouse Brain showing detection of ~14.46kDa Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~14.46kDa. Other Band (s): Higher molecular weight oligomers.

Western blot analysis of Human Recombinant Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW ladder. Lane 2: Alpha Synuclein. Lane 3: Beta Synuclein. Lane 4: Gamma Synuclein. Load: 0.5 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Alpha synuclein antibody shows cross-reactivity with beta synuclein but not gamma synuclein.

Western blot analysis of Human Truncated Alpha Synuclein Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: hASYN aa 1-114 (5 uL). Load: 5 uL. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.