alpha Actinin 4 antibody [N2C1], Internal

• Catalog Number: orb556835
• Category: Antibodies
• Description: Rabbit polyclonal antibody to alpha Actinin 4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 4. The exact sequence is proprietary.
• Concentration: 1.03 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 105
• Target: alpha Actinin 4
• UniProt ID: O43707
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: actinin alpha 4 , ACTININ-4 , FSGS , FSGS1
• Note: For research use only
• Application notes: Positive control: Mouse brain , PC-12 , A431 , HepG2 , NIH-3T3
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using alpha Actinin 4 antibody

Immunohistochemical staining of A549 xenograft using alpha Actinin 4 antibody

Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody

Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody

Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody

Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody