Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

• Catalog Number: orb749772
• Category: Antibodies
• Description: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1A4 + ACTA2/791
• Tested applications: IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG2a, kappa
• Immunogen: An N-terminal decapeptide of the alpha smooth muscle isoform of Actin (1A4) and recombinant full-length human protein (ACTA2/791) were used as the immunogen for the ACTA2 antibody cocktail.
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This ACTA2 antibody cocktail is available for research use only.
• UniProt ID: P62736
• Storage: Store the ACTA2 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ACTA2 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

IHC: Formalin-fixed, paraffin-embedded rat lung stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

IHC: Formalin-fixed, paraffin-embedded rat stomach stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

Immunofluorescent staining of fixed human HeLa cells with ACTA2 antibody cocktail antibody (clone 1A4 + ACTA2/791, green) and NucSpot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free ACTA2 antibody cocktail antibody (clone 1A4 + ACTA2/791) as confirmation of integrity and purity.