Alpha Actin Antibody / ACTA2 (smooth muscle)

• Catalog Number: orb385893
• Category: Antibodies
• Description: Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SMAT2-1
• Tested applications: IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Smooth Muscle Actin antibody is available for research use only.
• UniProt ID: P62736
• Storage: Store the Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human angiosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human leiomyosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat lung with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human HeLa cells with Smooth Muscle Actin antibody (green, clone SMAT2-1) and Reddot nuclear stain (red).