Alpha-1-Trypsin antibody

• Catalog Number: orb344181
• Category: Antibodies
• Description: Alpha-1-Trypsin antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: a1-Anti-Trypsin [Human Plasma]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-ALPHA-1-ANTI-TRYPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: P01009
• NCBI: NP_000286.3
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Alpha-1-Trypsin Antibody, A1AT antibody,
• Note: For research use only
• Application notes: Anti-Alpha-1-Anti-trypsin Antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Alpha-1-Anti-trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested.
• Expiration Date: 12 months from date of receipt.

Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 at 30 min RT.

Western Blot of Goat anti Alpha-1 anti-Trypsin antibody. Lane 1: Alpha-1 anti-Trypsin under reducing conditions. Load: 1 µg per lane. Primary antibody: Alpha-1 anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: DyLight 488 conjugated Donkey anti-goat secondary antibody at 1:10000 for 45 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 55 kDa for α-1 Anti-Trypsin. Other band(s): none.

Western Blot of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 µg per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4°C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight™ 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated (blue) secondary antibody at 1:10000 for ~ 1hr at 4 degrees. Block: Blocking Buffer for Fluorescent Western Blotting - orb348637 overnight at 4°C.

Western Blot of Goat anti-Alpha-1-Anti-Trypsin antibody. Lane 1: Albumin depleted human serum. Load: ~10 µg per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody (p/n orb346990) at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none.