Alpha 1 Antitrypsin Antibody / AAT / SERPINA1

• Catalog Number: orb639863
• Category: Antibodies
• Description: The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: AAT/3167R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A recombinant human Alpha-1-Antitrypsin (AAT) partial protein was used as the immunogen for this recombinant Alpha 1 Antitrypsin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Alpha 1 Antitrypsin antibody is available for research use only.
• UniProt ID: P01009
• Storage: Store the recombinant Alpha 1 Antitrypsin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human samples with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).

SDS-PAGE analysis of purified, BSA-free recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R) as confirmation of integrity and purity.