Alpha-1-Anti-Trypsin Antibody

• Catalog Number: orb344180
• Category: Antibodies
• Description: Alpha-1-Trypsin antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: a1-Anti-Trypsin [Human Plasma]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-ALPHA-1-ANTI-TRYPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: P01009
• NCBI: NP_000286.3
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Alpha-1-Trypsin Antibody, A1AT antibody,
• Note: For research use only
• Application notes: Anti-Alpha-1-Anti-trypsin Antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Alpha-1-Anti-trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested.
• Expiration Date: 12 months from date of receipt.

Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 at 30 min RT.

Western Blot of Goat anti Alpha-1 anti-Trypsin antibody. Lane 1: Alpha-1 anti-Trypsin under reducing conditions. Load: 1 µg per lane. Primary antibody: Alpha-1 anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: DyLight 488 conjugated Donkey anti-goat secondary antibody at 1:10000 for 45 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 55kDa for α-1 Anti-Trypsin. Other band(s): none.

Western Blot of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 µg per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4°C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight™ 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated (blue) secondary antibody at 1:10000 for ~ 1hr at 4 degrees. Block: Blocking Buffer for Fluorescent Western Blotting - orb348637 overnight at 4°C

Western Blot of Goat anti-Alpha-1-Anti-Trypsin antibody. Lane 1: Albumin depleted human serum. Load: ~10 µg per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody (p/n orb346990) at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none