ALOX5 Antibody

• Catalog Number: orb1273560
• Category: Antibodies
• Description: ALOX5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Rat
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-lipoxygenase (5-LO).
• Concentration: batch dependent
• Dilution range: The antibody has been directly tested for reactivity in Western blots with rat and human tissue. It is anticipated that the antibody will react with non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 80
• Target: ALOX5
• UniProt ID: P09917
• NCBI: P09917
• Storage: 5 Lipoxygenase antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
• Note: For research use only
• Application notes: The antibody has been directly tested for reactivity in Western blots with rat and human tissue. It is anticipated that the antibody will react with non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Expiration Date: 12 months from date of receipt.

Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with lamda-Ptase.