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Catalog Number | orb1257142 |
---|---|
Category | Antibodies |
Description | ALKBH1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 44kDa |
Target | ALKBH1 |
UniProt ID | Q13686 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | ABH, ABH1, ALKBH, alkB, hABH, nucleic acid dioxyge Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ALKBH1 antibody (orb1257142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug ALKBH1 antibody (orb1257142). Western blot was performed from the immunoprecipitate using ALKBH1 antibody (orb1257142) at a dilution of 1:1000.
IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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