Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]

• Catalog Number: orb1675047
• Category: Antibodies
• Description: Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]
• Species/Host: Goat
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Donkey
• Immunogen: Donkey IgG whole molecule
• Concentration: 0.59 mg/ml (by UV absorbance at 280 nm)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Donkey IgG, and Donkey Serum.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
• Buffer/Preservatives: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Note: For research use only
• Application notes: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:16,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.