Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Rabbit]

• Catalog Number: orb1675046
• Category: Antibodies
• Description: Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Rabbit]
• Species/Host: Rabbit
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Donkey
• Immunogen: Donkey IgG whole molecule
• Concentration: 0.9 mg/ml (by UV absorbance at 280 nm)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Donkey IgG and Donkey Serum.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
• Buffer/Preservatives: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Note: For research use only
• Application notes: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate code # NPP-10) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.