ALG1 Antibody

• Catalog Number: orb1256163
• Category: Antibodies
• Description: ALG1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 60kDa
• Target: ALG1
• UniProt ID: Q9BT22
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: ALG1, HMAT1, HMT-1, HMT1, MT-1, CDG1K, Mat-1, hMat
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ALG1 antibody (orb1256163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of A549 cells using ALG1 antibody (orb1256163). Blue: DAPI for nuclear staining.