ALDH1L1 Antibody

• Catalog Number: orb1823644
• Category: Antibodies
• Description: Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde, lipid peroxidation and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH1L1 (aldehyde dehydrogenase 1 family member L1), also known as FTHFD or 10-FTHFD (10-formyltetrahydrofolate dehydrogenase), is a cytosolic protein that is developmentally regulated in the cerebellum. ALDH1L1 binds to folate and catalyzes the conversion of 10-formyltetrahydrofolate (10-FTHF) to tetrahydrofolate (THF). This suggests a possible role for ALDH1L1 in the regulation of cellular THF levels as well as in the inhibition of cell proliferation (as 10-FTHF is essential for the synthesis of purine). In addition, the overexpression of ALDH1L1 can restrict cell proliferation in vitro.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ALDH1L1/7702
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant fragment of human ALDH1L1 protein was used as the immunogen for the ALDH1L1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ALDH1L1 antibody is available for research use only.
• UniProt ID: O75891
• Storage: Aliquot the ALDH1L1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human hepatocellular carcinoma tissue with ADH1L1 antibody (clone ALDH1L1/7702). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon tissue with ADH1L1 antibody (clone ALDH1L1/7702). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free ADH1L1 antibody (clone ALDH1L1/7702) as confirmation of integrity and purity.