Aldehyde Dehydrogenase antibody (Peroxidase)

• Catalog Number: orb344871
• Category: Antibodies
• Description: Aldehyde Dehydrogenase antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Yeast
• Isotype: IgG
• Immunogen: Aldehyde Dehydrogenase [Yeast]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.
• Conjugation: HRP
• UniProt ID: P47771
• NCBI: NP_013893.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Aldehyde Dehydrogenase Antibody Peroxi
• Note: For research use only
• Application notes: Anti-Aldehyde Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Aldehyde Dehydrogenase (yeast) Antibody Peroxidase Conjugated. Lane 1: Aldehyde Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Aldehyde Dehydrogenase (yeast) Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: n/a. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Aldehyde Dehydrogenase (yeast).