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Catalog Number | orb381031 |
---|---|
Category | Antibodies |
Description | Alcohol Dehydrogenase/ADH1A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human ADH1A recombinant protein (Position: K213-F375). Human ADH1A shares 87.1% and 82.8% amino acid (aa) sequence identity with mouse and rat ADH1A, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 39859 MW |
UniProt ID | P07327 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Alcohol dehydrogenase 1A;1.1.1.1;Alcohol dehydroge Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-ADH1A antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of ADH1A using anti-ADH1A antibody.Lane 1:human HCCT tissue; 2:human HCCP tissue; 3:rat liver tissue.4:rat kidney tissue.5:mouse liver tissue.6:mouse kidney tissue.
IF analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in immunocytochemical section of U20S cells.
IHC analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in a paraffin-embedded section of human liver cancer tissue.
IHC analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in a paraffin-embedded section of mouse liver tissue.
IHC analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in a paraffin-embedded section of rat liver tissue.
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