Alcohol Dehydrogenase antibody

• Catalog Number: orb344798
• Category: Antibodies
• Description: Alcohol Dehydrogenase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Yeast
• Isotype: IgG
• Immunogen: Alcohol Dehydrogenase [Yeast]
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P00330
• NCBI: 12643994
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Alcohol Dehydrogenase Antibody, Alcoho
• Note: For research use only
• Application notes: Anti-Alcohol Dehydrogenase has been tested by western blot and is suitable for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Alcohol Dehydrogenase Antibody. Lane 1: Alcohol Dehydrogenase. Load: 50 ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 Blocking Buffer for 30 min at RT. Predicted: 37 kDa/Observed size: 45 kDa.