AKT1 Antibody

• Catalog Number: orb699554
• Category: Antibodies
• Description: Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: AKT1/3898R
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.
• Dilution range: Western blot: 1-2ug/ml,ELISA: order Ab without BSA for coating
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant AKT1 antibody is available for research use only.
• UniProt ID: P31749
• Storage: Store the recombinant AKT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human MCF7 cell lysate with recombinant AKT1 antibody. Predicted molecular weight ~56 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant AKT1 antibody (clone AKT1/3898R) as confirmation of integrity and purity.