AKT1 Antibody

• Catalog Number: orb379635
• Category: Antibodies
• Description: Mouse monoclonal antibody to AKT1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1F7E10
• Tested applications: ELISA, FC, ICC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.
• Concentration: 1mg/ml
• Dilution range: WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; RCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 55.7kDa
• Entrez: 207
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of AKT1 antibody

Flow cytometric analysis of Hela cells using AKT1 antibody

Immunofluorescence analysis of HepG2 cells using AKT1 antibody

Western blot analysis of MCF-7 (Lane 1), NIH/3T3 (Lane 2), Hela (Lane 3), COS7 (Lane 4), and C6 (Lane 5) cell lysate using AKT1 antibody

Western blot analysis of HEK293 cell lysate using AKT1 antibody

Western blot analysis of recombinant protein using AKT1 antibody