AKT1 Antibody

• Catalog Number: orb1253810
• Category: Antibodies
• Description: AKT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Akt (Phospho-Ser473) antibody was raised against a peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60 kDa
• Target: AKT1
• UniProt ID: P31749
• NCBI: NP_001014431.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA,
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF, Serum or both using Akt (Phospho-Ser473).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473).