AKT1 Antibody

• Catalog Number: orb1253809
• Category: Antibodies
• Description: AKT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Akt (Phospho-Thr308) antibody was raised against a peptide sequence around phosphorylation site of threonine 308 (M-K-T (p) -F-C) derived from Human Akt.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60 kDa
• Target: AKT1
• UniProt ID: P31749
• NCBI: NP_001014431.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA,
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, serum or both using Akt (Phospho-Thr308).

Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Akt (Phospho-Thr308).