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Catalog Number | orb1253702 |
---|---|
Category | Antibodies |
Description | AKT1 AKT2 AKT3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y (p) -L-A) derived from Human AKT1/AKT2/AKT3. |
Concentration | 1 mg/mL |
Dilution range | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 60 kDa |
Target | AKT1 AKT2 AKT3 |
UniProt ID | Q9Y243, P31751, P31749 |
NCBI | NP_005456.1, NP_001617.1, NP_001014431.1 |
Storage | Store antibody at -20°C for up to one year. |
Buffer/Preservatives | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Alternative names | AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, Read more... |
Note | For research use only |
Application notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).
IF, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Primate, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Primate, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IH, IP, WB | |
Hamster, Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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