AKT (phospho-Y474) antibody

• Catalog Number: orb304680
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AKT (phospho-Y474)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat, Sheep, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y474 of human AKT protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: AKT1
• Entrez: 207, 24185, 11651
• UniProt ID: P47196, P31750, P31749
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PKB antibody, anti RAC antibody, anti RAC-alp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AKT (Phospho-Y474) expression in zebrafish (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)

Immunohistochemical analysis of AKT (Phospho-Y474) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.