AKR1A1 Antibody

• Catalog Number: orb1260347
• Category: Antibodies
• Description: AKR1A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human AKR1A1 (NP_006057.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 37kDa
• Target: AKR1A1
• UniProt ID: P14550
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: AKR1A1, aldo-keto reductase family 1, member A1 (a
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using AKR1A1 antibody (orb1260347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat kidney using AKR1A1 antibody (orb1260347) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using AKR1A1 antibody (orb1260347) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using AKR1A1 antibody (orb1260347).