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Catalog Number | orb1238078 |
---|---|
Category | Antibodies |
Description | AIMF2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Equine, Gorilla, Primate |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-AIFM2 antibody ( orb1238078) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AIFM2. The immunogen is located within the last 50 amino acids of AIFM2. |
Concentration | 1 mg/mL |
Dilution range | WB: 2 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human mouse and rat samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 41kDObserved: 41 kD |
Target | AIMF2 |
UniProt ID | Q9BRQ8 |
NCBI | Q9BRQ8 |
Storage | AIMF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | AIMF2 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Mouse Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Rat Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
Immunofluorescence Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with orb1238078 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of AIFM2 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Mouse Spleen. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
ELISA, IF, IHC, WB | |
Bovine, Equine, Gorilla, Primate | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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