AIF1 antibody

• Catalog Number: orb2276292
• Category: Antibodies
• Description: AIF1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-955M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2c, kappa
• Immunogen: Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P55008
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Allograft inflammatory factor 1 (AIF1); balloon an
• Note: For research use only
• Application notes: Positive Control: Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages. Negative Control: Colon: AIF1 immunostaining should be absent in all non-inflammatory cells. Cellular Localization: Cytoplasmic and cell surface. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Duodenum mucosa A strong AIF1 positivity is seen in mucosal macrophages of the duodenum.

Malignant mesothelioma epitheloid subtype containing numerous AIF1 positive inflammatory cells.

Placenta early AIF1 expression is particularly strong in macrophages of the placenta.