AGO3 Antibody / Argonaute-3 / eIF2C3

• Catalog Number: orb2636539
• Category: Antibodies
• Description: This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as Argonaute RISC catalytic component 3. Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5-flanking sequences to the region of guide-target complementarity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-AGO3-1C5
• Tested applications: ELISA, FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human AGO3 protein was used as the immunogen for the AGO3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This AGO3 antibody is available for research use only.
• UniProt ID: Q9H9G7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the AGO3 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells. Cytoplasmic P-bodies and nucleoplasm show staining with AGO3 antibody (green, clone PCRP-AGO3-1C5), and phalloidin counterstain (red).

FACS staining of PFA-fixed human HeLa cells with AGO3 antibody (blue, clone PCRP-AGO3-1C5) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free AGO3 antibody (clone PCRP-AGO3-1C5) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using AGO3 antibody (clone PCRP-AGO3-1C5). These results demonstrate the foremost specificity of the PCRP-AGO3-1C5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.