AGO2 (N2) Antibody, Rabbit Polyclonal

• Catalog Number: orb67055
• Category: Antibodies
• Description: Rabbit polyclonal to AGO2.Protein argonaute-2 (AGO2) is also known as Eukaryotic translation initiation factor 2C 2 (EIF2C2),which is required for RNA-mediated gene silencing NAi) by the RNA-induced silencing complexISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as amicroRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC tocomplementary mRNAs that are targets for RISC-mediated gene silencing. Binding of RISC to aperfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of themRNA specifically by EIF2C2/AGO2. The inhibition of translational initiation leads to the accumulationof the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation maysubsequently occur.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: N-terminal region of human AGO2
• Dilution range: WB:1:1000-1:5000
• Purity: Affinity purification
• Conjugation: Unconjugated
• MW: 97
• UniProt ID: Q9UKV8
• Storage: -20°C
• Buffer/Preservatives: 0.01% NaN3
• Alternative names: anti-AGO2 antibody,anti-EIF2C2 antibody,anti-Prote
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of total cell extracts from human hela(Lane1) and mouse MEF(Lane2) using AGO2 antibody