You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1672138 |
---|---|
Category | Antibodies |
Description | AFP Antibody |
Clonality | Recombinant |
Clone Number | AF5 |
Tested applications | FC, IF, WB |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | AF5 was prepared by immunizing female BALB/c mice with human amniotic fluid containing AFP, from a pregnancy affected by a neural tube defect, diluted and emulsified in Freund's complete adjuvant. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | AFP |
UniProt ID | P02771 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | A-Feto-protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow-cytometry using the anti-Alpha-fetoprotein AF5. HepG2 cells were stained with unimmunized r antibody (black line) or the rmeric version of AF5 (orb1672138 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
Western Blot using anti-Alpha-fetoprotein antibody AF5. HepG2 cell lysate (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of AF5 (orb1672138) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa- tho ugh this protein is also glycosylated (Uniprot ID: P02771). orb1672138 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.
Immunofluoresence staining of fixed HepG2 cells with anti-Alpha-fetoprotein (AFP) antibody AF5. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells- permewith 0.15% Triton stained with the chimeric r version of AF5 (orb1672138) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672138- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
ELISA, IF, IHC-P, WB | |
Bovine, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
Filter by Rating