AFG3L2 Antibody

• Catalog Number: orb371649
• Category: Antibodies
• Description: AFG3L2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human AFG3L2 recombinant protein (Position: R168-D250). Human AFG3L2 shares 100% amino acid (aa) sequence identity with mouse AFG3L2.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 88584 MW
• UniProt ID: Q9Y4W6
• Sensitivity: > 5000 cells
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: AFG3-like protein 2;3.4.24.-;Paraplegin-like prote
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-AFG3L2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of AFG3L2 using anti-AFG3L2 antibody.Lane 1:human HeLa cell; 2:human MCF-7 cell; 3:human Jurkat cell; 4:human MDA-MB-453 cell; 5:rat brain tissue; 6:rat liver tissue; 7:mouse brain tissue; 8:mouse liver tissue.

IF analysis of AFG3L2 using anti-AFG3L2 antibody. AFG3L2 was detected in immunocytochemical section of U20S cells.