AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated

• Catalog Number: orb608184
• Category: Antibodies
• Description: AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-Fr, IHC-P, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Native Rabbit IgG
• Concentration: 1.0 mg/ml
• Dilution range: WB=1:5000-20000, IHC-P=1:3000-5000, IHC-F=1:3000-5000, ELISA=1:5000-20000
• Conjugation: HRP
• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
• Alternative names: AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, M
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (orb608184) at 1/200 dilution and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (orb608184) at 1/200 dilution and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (orb608184) at 1/200 dilution and DAB staining.