Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

• Catalog Number: orb606688
• Category: Antibodies
• Description: Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ADFP/2755R
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amin acids 249-376 from the human protein were used as the immunogen for this recombinant Adipophilin antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Adipophilin antibody is available for research use only.
• UniProt ID: Q99541
• Storage: The recombinant Adipophilin antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant Adipophilin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human adrenal tissue with recombinant Adipophilin antibody (clone ADFP/2755R). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

Western blot testing of human liver lysate with recombinant Adipophilin antibody. Predicted molecular weight ~48 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Adipophilin antibody (clone ADFP/2755R) as confirmation of integrity and purity.