ACVRL1 Antibody (N-term)

• Catalog Number: orb1928871
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB03736
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 56124 Da
• Target: This ACVRL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-68 amino acids from the N-terminal region of human ACVRL1.
• UniProt ID: P37023
• NCBI: NP_000011.2, NP_001070869.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Serine/threonine-protein kinase receptor R3, SKR3,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot of ACVRL1 Pab. TOP LEFT: Mouse heart tissue lysate.

Flow cytometric analysis of HepG2 cells using ACVRL1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ACVRL1 (arrow) using rabbit polyclonal ACVRL1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2).

human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1 (lane 2, 4). RIPA lysis buffer, 20 ug/lane of protein, primary antibody dilution 1:1000, blocking solution is 5% milk in TBST (lane 1 and 2), 5% BSA in TBST (lane 3 and 4).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.