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Catalog Number | orb1928870 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB03737 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 56124 Da |
Target | This ACVRL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human ACVRL1. |
UniProt ID | P37023 |
NCBI | NP_000011.2, NP_001070869.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Serine/threonine-protein kinase receptor R3, SKR3, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of hACVRL1-L489 in Jurkat (lane 1), HepG2 (lane 2) cell line lysates (35 ug/lane). ACVRL1 (arrow) was detected using the purified Pab.
ACVRL1 Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.