ACTN2 Antibody / Alpha Actinin 2 / Sarcomeric Alpha Actinin

• Catalog Number: orb1824158
• Category: Antibodies
• Description: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ACTN2/8184R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portin of amino acids 557-692 from the human protein was used as the immunogen for the Sarcomeric Actinin Alpha 2 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This Sarcomeric Actinin Alpha 2 antibody is available for research use only.
• UniProt ID: P35609
• Storage: Aliquot the Sarcomeric Actinin Alpha 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human skeletal muscle tissue with ACTN2 antibody (clone ACTN2/8184R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Sarcomeric Actinin Alpha 2 antibody (clone ACTN2/8184R) as confirmation of integrity and purity.