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Catalog Number | orb1244248 |
---|---|
Category | Antibodies |
Description | ACTN1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 1. |
Concentration | batch dependent |
Dilution range | Actin alpha 1 antibody can be used for detection of Actin alpha 1 by ELISA at 1:312500. Actin alpha 1 antibody can be used for detection of Actin alpha 1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 103 kDa |
Target | ACTN1 |
UniProt ID | P12814 |
NCBI | NP_001093 |
Storage | For short periods of storage (days) store at 4°C. For longer periods of storage, store Actin alpha 1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles. |
Buffer/Preservatives | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Alternative names | ACTN1, FLJ40884, BDPLT15 Read more... |
Note | For research use only |
Application notes | Actin alpha 1 antibody can be used for detection of Actin alpha 1 by ELISA at 1:312500. Actin alpha 1 antibody can be used for detection of Actin alpha 1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
Expiration Date | 12 months from date of receipt. |
Antibody used in WB on Rat Cortical Neurons at 1:1000.
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Antibody used in WB on Human HEK293 at 1:1000.
Antibody used in WB on rACTNX-GFP at 1:1000 (Lane 1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane 2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane 3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane 4: 10 ug ACTN4-GFP transfected COS-7 lysate).
Antibody used in IHC on rACTNX-GFP at 1:1000.
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Zebrafish | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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