Actin (smooth muscle) antibody

• Catalog Number: orb1151555
• Category: Antibodies
• Description: Mouse monoclonal antibody to Actin (smooth muscle)
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rACTA2/1625
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin was used as the immunogen for the Smooth Muscle Actin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Smooth Muscle Actin antibody is available for research use only.
• UniProt ID: P62736
• Storage: Aliquot the Smooth Muscle Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human uterus tissue with Smooth Muscle Actin (clone rACTA2/1625). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skeletal muscle with Smooth Muscle Actin antibody (clone rACTA2/1625). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Smooth Muscle Actin antibody (rACTA2/1625) as confirmation of integrity and purity.